Confirmation of the Mechanisms of Resistance to ACCase-Inhibiting Herbicides in Chinese Sprangletop (Leptochloa chinensis (L.) Nees) from South Sulawesi, Indonesia

Chinese sprangletop (Leptochloa chinensis (L.) Nees) is recognized as the most disturbing weed in rice fields Africa, Australia, and Asia due to causing a significant reduction yields. The habit of lowland farmers Indonesia using high doses herbicides more than once without any rotation, leading increased resistance potential. Therefore, this study aimed confirm level L. acetyl-CoA carboxylase (ACCase)-inhibiting whole-plant pot test method. We identified other that can control resistant biotype performed DNA sequencing on sample determine mutations present biotype′s ACCase gene through polymerase chain reaction. herbicide dose–response experiment showed from Bantimurung Subdistrict, Maros Regency, South Sulawesi, exhibited inhibitors (metamifop cyhalofop-butyl), which first case reported Indonesia. An acetolactate synthase inhibitor (bispyribac-sodium) 1-deoxy-d-xylulose-5-phosphate (DOXP) (clomazone) were effective at controlling biotypes chinensis, so could considered for use rotation or an ingredient mixed herbicides. Single-nucleotide substitution guanine thiamine position 6081 (TGG; susceptible, TGT; Maros) causes Trp2027Cys mutation target contributes inhibitors.